Architecture Timeline of Important Historic Periods

Architecture Timeline of Important Historic Periods When did Western architecture begin? Long before the magnificent structures of ancient Greece and Rome, humans were designing and constructing. The period known as the Classical Era grew from ideas and construction techniques that evolved centuries and eons apart in distant locations. This review illustrates how each new movement builds on the one before. Although our timeline lists dates related mostly to American architecture, historic periods do not start and stop at precise points on a map or a calendar. Periods and styles flow together, sometimes merging contradictory ideas, sometimes inventing new approaches, and often re-awakening and re-inventing older movements. Dates are always approximate - architecture is a fluid art. 11,600 B.C. to 3,500 B.C. - Prehistoric Times Stonehenge in Amesbury, United Kingdom. Jason Hawkes/Getty Images Archaeologists dig prehistory. Gà ¶bekli Tepe in present day Turkey is a good example of archaeological architecture. Before recorded history, humans constructed earthen mounds, stone circles, megaliths, and structures that often puzzle modern-day archaeologists. Prehistoric architecture includes monumental structures such as Stonehenge, cliff dwellings in the Americas, and thatch and mud structures lost to time. The dawn of architecture is found in these structures. Prehistoric builders moved earth and stone into geometric forms, creating our earliest human-made formations. We dont know why primitive people began building geometric structures. Archaeologists can only guess that prehistoric people looked to the heavens to imitate the sun and the moon, using that circular shape in their creations of earth mounds and monolithic henges. Many fine examples of well-preserved prehistoric architecture are found in southern England. Stonehenge in Amesbury, United Kingdom is a well-known example of the prehistoric stone circle. Nearby Silbury Hill, also in Wiltshire, is the largest man-made, prehistoric earthen mound in Europe. At 30 meters high and 160 meters wide, the gravel mound is layers of soil, mud, and grass, with dug pits and tunnels of chalk and clay. Completed in the late Neolithic period, approximately 2,400 BC, its architects were a Neolithic civilization in Britain. The prehistoric sites in southern Britain (Stonehenge, Avebury, and associated sites) are collectively a UNESCO World Heritage Site. The design, position, and inter-relationship of the monuments and sites, according to UNESCO, are evidence of a wealthy and highly organized prehistoric society able to impose its concepts on the environment. To some, the ability to change the environment is key for a structure to be called architecture. Prehistoric structures are sometimes considered the birth of architecture. If nothing else, primitive structures certainly raise the question, what is architecture? Why does the circle dominate mans earliest architecture? It is the shape of the sun and the moon, the first shape humans realized to be significant to their lives. The duo of architecture and geometry goes way back in time and may be the source of what humans find beautiful even today. 3,050 B.C. to 900 B.C. - Ancient Egypt The Pyramid of Khafre (Chephren) in Giza, Egypt. Lansbricae (Luis Leclere)/Getty Images (cropped) In ancient Egypt, powerful rulers constructed monumental pyramids, temples, and shrines. Far from primitive, enormous structures such as the Pyramids of Giza were feats of engineering capable of reaching great heights. Scholars have delineated the periods of history in ancient Egypt. Wood was not widely available in the arid Egyptian landscape. Houses in ancient Egypt were made with blocks of sun-baked mud. Flooding of the Nile River and the ravages of time destroyed most of these ancient homes. Much of what we know about ancient Egypt is based on great temples and tombs, which were made with granite and limestone and decorated with hieroglyphics, carvings, and brightly colored frescoes. The ancient Egyptians didnt use mortar, so the stones were carefully cut to fit together. The pyramid form was a marvel of engineering that allowed ancient Egyptians to build enormous structures. The development of the pyramid form allowed Egyptians to build enormous tombs for their kings. The sloping walls could reach great heights because their weight was supported by the wide pyramid base. An innovative Egyptian named Imhotep is said to have designed one of the earliest of the massive stone monuments, the Step Pyramid of Djoser (2,667 B.C. to 2,648 B.C.). Builders in ancient Egypt didnt use load-bearing arches. Instead, columns were placed close together to support the heavy stone entablature above. Brightly painted and elaborately carved, the columns often mimicked palms, papyrus plants, and other plant forms. Over the centuries, at least thirty distinct column styles evolved. As the Roman Empire occupied these lands, both Persian and Egyptian columns have influenced Western architecture. Archaeological discoveries in Egypt reawakened an interest in the ancient temples and monuments. Egyptian Revival architecture became fashionable during the 1800s. In the early 1900s, the discovery of King Tuts tomb stirred a fascination for Egyptian artifacts and the rise of Art Deco architecture. 850 B.C. to A.D. 476 - Classical The Pantheon, A.D. 126, Rome, Italy. Werner Forman Archive/Heritage Images/Getty Images (cropped) Classical architecture refers to the style and design of buildings in ancient Greece and ancient Rome. Classical architecture shaped our approach to building in Western colonies around the world. From the rise of ancient Greece until the fall of the Roman empire, great buildings were constructed according to precise rules. The Roman architect Marcus Vitruvius, who lived during first century B.C., believed that builders should use mathematical principles when constructing temples. For without symmetry and proportion no temple can have a regular plan, Vitruvius wrote in his famous treatise De Architectura, or Ten Books on Architecture. In his writings, Vitruvius introduced the Classical orders, which defined column styles and entablature designs used in Classical architecture. The earliest Classical orders were Doric, Ionic, and Corinthian. Although we combine this architectural era and call it Classical, historians have described these three Classical periods: 700 to 323 B.C. - Greek. The Doric column was first developed in Greece and it was used for great temples, including the famous Parthenon in Athens. Simple Ionic columns were used for smaller temples and building interiors. 323 to 146 B.C. - Hellenistic. When Greece was at the height of its power in Europe and Asia, the empire built elaborate temples and secular buildings with Ionic and Corinthian columns. The Hellenistic period ended with conquests by the Roman Empire. 44 B.C. to A.D. 476 - Roman. The Romans borrowed heavily from the earlier Greek and Hellenistic styles, but their buildings were more highly ornamented. They used Corinthian and composite style columns along with decorative brackets. The invention of concrete allowed the Romans to build arches, vaults, and domes. Famous examples of Roman architecture include the Roman Colosseum and the Pantheon in Rome. Much of this ancient architecture is in ruins or partially rebuilt. Virtual reality programs like Romereborn.org attempt to digitally recreate the environment of this important civilization. 527 to 565 - Byzantine Church of Hagia Eirene in the First Courtyard of the TopkapÄ ± Palace, Istanbul, Turkey. Salvator Barki/Getty Images (cropped) After Constantine moved the capital of the Roman empire to Byzantium (now called Istanbul in Turkey) in A.D. 330, Roman architecture evolved into a graceful, classically-inspired style that used brick instead of stone, domed roofs, elaborate mosaics, and classical forms. Emperor Justinian (527 to 565) led the way. Eastern and Western traditions combined in the sacred buildings of the Byzantine period. Buildings were designed with a central dome that eventually rose to new heights by using engineering practices refined in the Middle East. This era of architectural history was transitional and  transformational. 800 to 1200 - Romanesque Romanesque Architecture of the Basilica of St. Sernin (1070-1120) in Toulouse, France. Anger O./AgenceImages courtesy Getty Images As Rome spread across Europe, heavier, stocky Romanesque architecture with rounded arches emerged. Churches and castles of the early Medieval period were constructed with thick walls and heavy piers. Even as the Roman Empire faded, Roman ideas reached far across Europe. Built between 1070 and 1120, the  Basilica of St. Sernin in Toulouse, France is a good example of this transitional architecture, with a Byzantine-domed apse and an added Gothic-like steeple. The floor plan is that of the Latin cross, Gothic-like again, with a high alter and tower at the cross intersection. Constructed of stone and brick, St. Sernin is on the pilgrimage route to Santiago de Compostela. 1100 to 1450 - Gothic The Gothic Cathedral of Notre Dame de Chartres, France. Alessandro Vannini/Getty Images (cropped) Early in the 12th century, new ways of building meant that cathedrals and other large buildings could soar to new heights. Gothic architecture became characterized by the elements that supported taller, more graceful architecture - innovations such as pointed arches, flying buttresses, and ribbed vaulting. In addition, elaborate stained glass could take the place of walls that no longer were used to support high ceilings. Gargoyles and other sculpting enabled practical and decorative functions. Many of the worlds most well-known sacred places are from this period in architectural history, including Chartres Cathedral and Paris Notre Dame Cathedral in France and Dublins St. Patricks Cathedral and Adare Friary in Ireland. Gothic architecture began mainly in France where builders began to adapt the earlier Romanesque style. Builders were also influenced by the pointed arches and elaborate stonework of Moorish architecture in Spain. One of the earliest Gothic buildings was the ambulatory of the abbey of St. Denis in France, built between 1140 and 1144. Originally, Gothic architecture was known as the French Style. During the Renaissance, after the French Style had fallen out of fashion, artisans mocked it. They coined the word Gothic to suggest that French Style buildings were the crude work of German (Goth) barbarians. Although the label wasnt accurate, the name Gothic remained. While builders were creating the great Gothic cathedrals of Europe, painters and sculptors in northern Italy were breaking away from rigid medieval styles and laying the foundation for the Renaissance. Art historians call the period between 1200 to 1400 the Early Renaissance or the Proto-Renaissance of art history. Fascination for medieval Gothic architecture was reawakened in the 19th and 20th centuries. Architects in Europe and the United States designed great buildings and private homes that imitated the cathedrals of medieval Europe. If a building looks Gothic and has Gothic elements and characteristics, but it was built in the 1800s or later, its style is Gothic Revival. 1400 to 1600 - Renaissance Villa Rotonda (Villa Almerico-Capra), near Venice, Italy, 1566-1590, Andrea Palladio. Massimo Maria Canevarolo via Wikimedia Commons, Creative Commons Attribution-ShareAlike 3.0 Unported (CC BY-SA 3.0) A return to Classical ideas ushered an age of awakening in Italy, France, and England. During the Renaissance era architects and builders were inspired by the carefully proportioned buildings of ancient Greece and Rome.  Italian Renaissance master Andrea Palladio helped awaken a passion for classical architecture when he designed beautiful, highly symmetrical villas such as Villa Rotonda near Venice, Italy. More than 1,500 years after the Roman architect Vitruvius wrote his important book, the Renaissance architect Giacomo da Vignola outlined Vitruviuss ideas. Published in 1563, Vignolas The Five Orders of Architecture became a guide for builders throughout western Europe. In 1570 Andrea Palladio used the new technology of movable type to publish I Quattro Libri dell Architettura, or The Four Books of Architecture. In this book, Palladio showed how Classical rules could be used not just for grand temples but also for private villas. Palladios ideas did not imitate the Classical order of architecture but his designs were in the manner of ancient designs. The work of the Renaissance masters spread across Europe, and long after the era ended, architects in the Western world would find inspiration in the beautifully proportioned architecture of the period. In the United States its descendant designs have been called neoclassical. 1600 to 1830 - Baroque The Baroque Palace of Versailles in France. Loop Images Tiara Anggamulia/Getty Images (cropped) Early in the 1600s, an elaborate new architectural style lavished buildings. What became known as Baroque was characterized by complex shapes, extravagant ornaments, opulent paintings, and bold contrasts. In Italy, the Baroque style is reflected in opulent and dramatic churches with irregular shapes and extravagant ornamentation. In France, the highly ornamented Baroque style combines with Classical restraint. Russian aristocrats were impressed by the Palace of Versailles, France and incorporated Baroque ideas in the building of St. Petersburg. Elements of the elaborate Baroque style are found throughout Europe. Architecture was only one expression of the Baroque style. In music, famous names included Bach, Handel, and Vivaldi. In the art world, Caravaggio, Bernini, Rubens, Rembrandt, Vermeer, and Velzquez are remembered. Famous inventors and scientists of the day include Blaise Pascal and Isaac Newton. 1650 to 1790 - Rococo Catherine Palace Near Saint Petersburg, Russia. Saravut Eksuwan/Getty Images During the last phase of the Baroque period, builders constructed graceful white buildings with sweeping curves. Rococo art and architecture is characterized by elegant decorative designs with scrolls, vines, shell-shapes, and delicate geometric patterns. Rococo architects applied Baroque ideas with a lighter, more graceful touch. In fact, some historians suggest that Rococo is simply a later phase of the Baroque period. Architects of this period include the great Bavarian stucco masters like Dominikus Zimmermann, whose  1750 Pilgrimage Church of Wies is a UNESCO World Heritage site. 1730 to 1925 - Neoclassicism The U.S. Capitol in Washington, D.C. Architect of the Capitol By the 1700s, European architects were turning away from elaborate Baroque and Rococo styles in favor of restrained Neoclassical approaches. Orderly, symmetrical Neoclassical architecture reflected the intellectual awakening among the middle and upper classes in Europe during the period historians often call the Enlightenment. Ornate Baroque and Rococo styles fell out of favor as architects for a growing middle class reacted to and rejected the opulence of the ruling class. French and American revolutions returned design to Classical ideals  - including equality and democracy - emblematic of the civilizations of ancient Greece and Rome. A keen interest in ideas of Renaissance architect Andrea Palladio inspired a return of Classical shapes in Europe, Great Britain, and the United States. These buildings were proportioned according to the classical orders with details borrowed from ancient Greece and Rome. In the late 1700s and early 1800s, the newly-formed United States drew upon Classical ideals to construct grand government buildings and an array of smaller, private homes. 1890 to 1914 - Art Nouveau The 1910 Hà ´tel Lutetia in Paris, France. Justin Lorget/chesnot/Corbis via Getty Images Known as the New Style in France, Art Nouveau was first expressed in fabrics and graphic design. The style spread to architecture and furniture in the 1890s as a revolt against industrialization turned peoples attention to the natural forms and personal craftsmanship of the Arts and Crafts Movement. Art Nouveau buildings often have asymmetrical shapes, arches, and decorative Japanese-like surfaces with curved, plant-like designs and mosaics. The period is often confused with Art Deco, which has an entirely different visual look and philosophical origin. Note that the name Art Nouveau is French, but the philosophy  - to some extent spread by the ideas of William Morris and the writings of John Ruskin - gave rise to similar movements throughout Europe. In Germany it was called Jugendstil; in Austria it was Sezessionsstil; in Spain it was Modernismo, which predicts or event begins the modern era. The works of Spanish architect Antoni Gaudà ­ (1852-1926) are said to be influenced by Art Nouveau or Modernismo, and Gaudi is often called one of the first modernist architects. 1895 to 1925 - Beaux Arts The Paris Opera by Beaux Arts Architect Charles Garnier. Francisco Andrade/Getty Images (cropped) Also known as Beaux Arts Classicism, Academic Classicism, or Classical Revival, Beaux Arts architecture is characterized by order, symmetry, formal design, grandiosity, and elaborate ornamentation. Combining classical Greek and Roman architecture with Renaissance ideas, Beaux Arts architecture was a favored style for grand public buildings and opulent mansions. 1905 to 1930 - Neo-Gothic The Neo-Gothic 1924 Tribune Tower in Chicago. Glowimage/Getty Images (cropped) In the early 20th century, medieval Gothic ideas were applied to modern buildings, both private homes and the new type of architecture called skyscrapers. Gothic Revival was a Victorian style inspired by Gothic cathedrals and other medieval architecture.  Gothic Revival home design began in the United Kingdom in the 1700s when Sir Horace Walpole decided to remodel his home, Strawberry Hill. In the early 20th century, Gothic Revival ideas were applied to modern skyscrapers, which are often called Neo-Gothic. Neo-Gothic skyscrapers often have strong vertical lines and a sense of great height; arched and pointed windows with decorative tracery; gargoyles and other medieval carvings; and pinnacles. The 1924 Chicago Tribune Tower is a good example of Neo-Gothic architecture. The architects Raymond Hood and John Howells were selected over many other architects to design the building. Their Neo-Gothic design may have appealed to the judges because it reflected a conservative (some critics said regressive) approach. The facade of the Tribune Tower is studded with rocks collected from great buildings around the world. Other Neo-Gothic buildings include the Cass Gilbert design for the Woolworth Building in New York City. 1925 to 1937 - Art Deco The Art Deco Chrysler Building in New York City. CreativeDream/Getty Images With their sleek forms and ziggurat designs, Art Deco architecture embraced both the machine age and ancient times. Zigzag patterns and vertical lines create dramatic effect on jazz-age, Art Deco buildings. Interestingly, many Art Deco motifs were inspired by the architecture of ancient Egypt. The Art Deco style evolved from many sources. The austere shapes of the modernist Bauhaus School and streamlined styling of modern technology combined with patterns and icons taken from the Far East, classical Greece and Rome, Africa, ancient Egypt and the Middle East, India, and Mayan and Aztec cultures. Art Deco buildings have many of these features: cubic forms; ziggurat, terraced pyramid shapes with each story smaller than the one below it; complex groupings of rectangles or trapezoids; bands of color; zigzag designs like lightening bolts; strong sense of line; and the illusion of pillars. By the 1930s, Art Deco evolved into a more simplified style known as Streamlined Moderne, or Art Moderne. The emphasis was on sleek, curving forms and long horizontal lines. These buildings did not feature zigzag or colorful designs found on earlier Art Deco architecture. Some of the most famous art deco buildings have become tourist destinations in New York City - the Empire State Building and Radio City Music Hall may be the most famous. The 1930 Chrysler Building in New York City was one of the first buildings composed of stainless steel over a large exposed surface. The architect, William Van Alen, drew inspiration from machine technology for the ornamental details on the Chrysler Building: There are eagle hood ornaments, hubcaps, and abstract images of cars. 1900 to Present - Modernist Styles De La Warr Pavilion, 1935, Bexhill on Sea, East Sussex, United Kingdom. Peter Thompson Heritage Images/Getty Images The 20th and 21st centuries have seen dramatic changes and astonishing diversity. Modernist styles have come and gone  - and continue to evolve. Modern-day trends include Art Moderne and the Bauhaus school coined by Walter Gropius, Deconstructivism, Formalism, Brutalism, and Structuralism. Modernism is not just another style  - it presents a new way of thinking. Modernist architecture emphasizes function. It attempts to provide for specific needs rather than imitate nature. The roots of Modernism may be found in the work of Berthold Luberkin (1901-1990), a Russian architect who settled in London and founded a group called Tecton. The Tecton architects believed in applying scientific, analytical methods to design. Their stark buildings ran counter to expectations and often seemed to defy gravity. The expressionistic work of the Polish-born German architect Erich Mendelsohn (1887-1953) also furthered the modernist movement. Mendelsohn and Russian-born English architect Serge Chermayeff (1900-1996) won the competition to design the De La Warr Pavilion in Britain. The 1935 seaside public hall has been called Streamline Moderne and International, but it most certainly is one of the first modernist buildings to be constructed and restored, maintaining its original beauty over the years. Modernist architecture can express a number of stylistic ideas, including Expressionism and Structuralism. In the later decades of the twentieth century, designers rebelled against the rational Modernism and a variety of Postmodern styles evolved. Modernist architecture generally has little or no ornamentation and is prefabricated or has factory-made parts. The design emphasizes function and the man-made construction materials are usually glass, metal, and concrete. Philosophically, modern architects rebel against traditional styles. For examples of Modernism in architecture, see works by Rem Koolhaas, I.M. Pei, Le Corbusier, Philip Johnson, and Mies van der Rohe. 1972 to Present - Postmodernism Postmodern Architecture at 220 Celebration Place, Celebration, Florida. Jackie Craven A reaction against the Modernist approaches gave rise to new buildings that re-invented historical details and familiar motifs. Look closely at these architectural movements and you are likely to find ideas that date back to classical and ancient times. Postmodern architecture evolved from the modernist movement, yet contradicts many of the modernist ideas. Combining new ideas with traditional forms, postmodernist buildings may startle, surprise, and even amuse. Familiar shapes and details are used in unexpected ways. Buildings may incorporate symbols to make a statement or simply to delight the viewer. Philip Johnsons ATT Headquarters is often cited as an example of postmodernism. Like many buildings in the International Style, the skyscraper has a sleek, classical facade. At the top, however, is an oversized Chippendale pediment. Johnsons design for the Town Hall in Celebration, Florida is also playfully over-the-top with columns in front of a public building. Well-known postmodern architects include Robert Venturi and Denise Scott Brown; Michael Graves; and the playful Philip Johnson, known for making fun of Modernism. The key ideas of Postmodernism are set forth in two important books by Robert Venturi. In his groundbreaking 1966 book, Complexity and Contradiction in Architecture, Venturi challenged modernism and celebrated the mix of historic styles in great cities such as Rome. Learning from Las Vegas, subtitled The Forgotten Symbolism of Architectural Form, became a postmodernist classic when Venturi called the vulgar billboards of the Vegas Strip emblems for a new architecture. Published in 1972, the book was written by Robert Venturi, Steven Izenour, and Denise Scott Brown. 1997 to Present - Neo-Modernism and Parametricism Zaha Hadids Heydar Aliyev Centre, 2012, Baku, Azerbaijan. Christopher Lee/Getty Images Throughout history, home designs have been influenced by the architecture du jour. In the not far off future, as computer costs come down and construction companies change their methods, homeowners and builders will be able to create fantastic designs. Some call todays architecture Neo-Modernism. Some call it Parametricism, but the name for computer-driven design is up for grabs. How did Neo-Modernism begin? Perhaps with Frank Gehrys sculpted designs, especially the success of the 1997 Guggenheim Museum in Bilbao, Spain. Maybe it began with architects who experimented with Binary Large Objects - BLOB architecture. But you might say that free-form design dates back to prehistoric times. Just look at Moshe Safdies 2011 Marina Bay Sands Resort in Singapore: It looks just like Stonehenge. Prehistoric Stonehenge (left) and Moshe Safdies 2011 Marina Bay Sands Resort in Singapore (right). Left: Grant Faint / Right: photo by william cho Sources History and Research: Silbury Hill, English Heritage Foundation, english-heritage.org.uk/daysout/properties/silbury-hill/history-and-research/; Stonehenge, Avebury and Associated Sites, UNESCO World Heritage Centre, United Nations, http://whc.unesco.org/en/list/373 Additional photo credits: Tribune Tower, Jon Arnold/Getty Images; Stonehenge / Marina Bay Sands Resort, Images (cropped) by Archive Photos/Archive Photos Collection/Getty Images (left) and AT Photography/Moment Collection/Getty Images (right)

Critical appraisal of a nursing quantitative study Essay

Critical appraisal of a nursing quantitative study - Essay Example is critical exposure of the nurse participants their levels of hygiene compliance despite work load and is important I self evaluation for personal competence improvements. The participants could also develop a better understanding of mediator roles of external factors on the nurses’ motivation and ability to comply with standards. This could then inform the nurses for self initiatives to managing the environmental factors towards higher level competence and compliance to hygiene standards and other standards. The major risk that the authors do not identify is possible psychological effects of the nurses knowledge of their lack of compliance because of the potential consequences of the non compliance to quality of delivered services. The authors are also silent on the process of recruiting research participants. There was however informed consent from research participants and administrator of the hospital facility that was used for data collection. Existence of informed consent further suggest voluntary participation by the involved nurses. It is however difficult to report possible approval by the institutional review board because the researchers do not report on it. Meeting minimum requirements for publication, such as ethical principles, however suggest that the authors ensured institutional review board approval besides informed consent and voluntary participation. The major variables are identified, though not explicitely, but are not defined. External factors, interms of work load, is the independent variable while staff compliance with hand hygiene standards is the dependent variable. personal observations and interviews were used for data collection and even though the researchers do not explain their rationale for choice of data collection methods, advantages such as convenience and unlimited access to information suggest the choice (Kothari, 2004). Data collection occurred over a one year period, beginning in June 2007 and ending in May 2008. It was

Development Essay Example | Topics and Well Written Essays - 500 words - 1

Development - Essay Example Therefore, private sector has become important in sparking development. However, the task is so overwhelming that it cannot be taken up by a single sector. Therefore, NGOs and charity groups have become very significant in ensuring that development is achieved. Different countries are willing to initiate development in various parts of the world. However, the private sector cannot be entrusted with monitoring how the funds are being utilized. As a result, non-governmental organizations have been given this mandate because they have specialized in ensuring that the development funds reach the right people. However, private sector cannot be left behind in development matters (De & Yehoue 2013). It has been instrumental in identifying areas that need more attention in order to achieve any form of development. In addition, it has been in the forefront in ensuring grants given to the government to start development projects are utilized effectively for the benefits of all people. Private sector partnership with charity groups and NGOs would enhance efficiency. All these parties have a single agenda of initiating development in the society. Therefore, once they bring their ideas and resources together, they would be able to achieve prosperity (Hodge, Greve, & Boardman 2010). This is through closing the gaps that existed which were being used by the elite group and politicians to embezzle funds that were meant for development. Content knowledge in topics such as climate change, financial access and last mile delivery provides non-governmental organizations with an advantage in assessing the needs of the community, policy implications, and customized solutions for specialized contexts (Desai & Potter 2002). Moreover, decades of experience by the non-governmental organizations which have been working with communities is significant in integrating cultural awareness, trusted relationship, and

Determination of Protein Concentration Experiment

Determination of Protein Concentration Experiment ABSTRACT: The objective of this experiment is to determine the concentrations of RNase H which was purified in the previous lab experiment and of an unknown solution, which was administered by the TA. A Bradford reagent was utilized to determine the total concentration which binds to the protein. The samples were placed in a spectrophotometer and the absorbance was recorded for each sample. The data was compared to the calibration curve made using the standard protein solutions and the absorbance reading. Our sample was unknown #3, which had a total concentration of 1.418 mg/mL. The concentrations for the original flow through, washing buffer flow through, and the eluting buffer flow through were 0.021 mg/mL, 0.0274 mg/mL, and 0.014 mg/mL, respectively, with a 98% confidence interval of  ± 0.0021 %. INTRODUCTION: In the previous lab experiment, the His-tag protein RNase H was purified by implementing a method called affinity chromatography. Affinity chromatography is utilized to isolate and purify proteins due to its high selectivity to the protein of interest (Biochemistry, 2015). The unused eluting buffer, original flow through, eluting buffer flow through, and the washing buffer flow through were all retained and stored for the latter experiment #7. In addition to the solutions mentioned, five standards protein solutions are prepared by diluting a 1.56 mg/mL of bovine gamma globulin solution (IgG) and unused eluting buffer. The final prepared solution is placed in a spectrophotometer and set at an absorbance of 596 nm. The unused eluting buffer is used as the control in the experiment to balance the spectrophotometer. A calibration curve is created using the absorbance measured from the five standards. In most cases, the calibration curve or standard curves are generated using a least two sets of data or replicates, which holds true in our case. However, the average of the two absorbance sets were used to create the standard curve. The blank/control consists of a buffer without addition of protein. The protein standards have a known concentration of protein, and the unknown sample is the solution to be assayed (Lab Manual). All of the 20 prepared solutions contain the Bradford protein assay. Bovine gamma globulin is a protein assay that is used as a protein concentration reference standard for use in the Bradford for total protein assay. It is used in Bradford and other protein assays. The Bradford protein assay, also referred to as Bradford reagent, is commonly used in laboratories to determine the concentration of the protein within the sample. The reagent binds to the proteins present. The amount of protein present is proportional the binding of the Bradford reagent. Meaning that the more protein present in the sample, the greater the dye will bind. The reagent is said to colorimetric, therefore, a color change can be observed, in reference to the protein concentration. The blue hue from the reagent becomes progressively darker as we increase the protein concentration. The opposite holds true for the lighter blue hue, which indicates less protein is present in the solution. With the aid of the Bradford Reagent, it is possible to determine the total amount of protein c oncentration present in the sample of interest. EXPERIMENTAL PROCEDURES: MATERIALS: Unused Eluting Buffer (Control)-from lab #6 Eluting Buffer flow through (EB)-from lab #6 Washing Buffer flow through (WB)-from lab #6 Original Flow through (OFT)-from lab #6 1 mL of a 1.56 mg/mL Solution of Bovine Gamma Globulin (IgG) 0.25, 0.50, 0.75, 1.0, and 1.5 mg/mL of IgG-Protein Concentration Standards Bradford Reagent 20 plastic test tubes Cuvets, disposable plastic Parafilm Spectrophotometer PROCEDURES: Prepare five standard protein solutions in a microcentrifuge tube by diluting the 1.56 mg/mL of IgG stock solution appropriately with unused eluting buffer from lab #6. Each solution should have a total volume of 250  µL and the concentrations should be as follows: 0.25, 0.5, 0.75, 1.0, and 1.5 of IgG. Set up 20 test tubes and divide into two sets. Label 5 test tubes per the concentrations mentioned above, and the remaining 5 tubes as follows; eluting buffer flow through, unused eluting buffer, original flow through, washing flow through, and the unknown sample # obtained from the TA. Repeat this procedure for set two. Add 50  µL of the appropriate solution to each tube. Add 1.5 mL of Bradford reagent to each of the 20 test tubes then cover with each tube with parafilm and mix using the vortex mixer for approx..3 seconds. Wait 10 minutes and then transfer all the solutions to cuvets. For set one and two, place the unused eluting buffer cuvet into the spectrophotometer a pparatus to balance and set to 596 nm. Read the absorbance for the other cuvets. Record each reading in lab notebook. Plot the average absorbance on the y-axis vs. concentration on the x-axis of the duplicate standard protein solutions. Determine the concentrations of the original flow through, washing flow through, eluting buffer flow through, and the unknown sample by using equation generated from the slope of the plot. RESULTS: DATA TABLES: Standard Protein Solutions: Protein Concentration (mg/mL) Volume of 1.56 mg/mL IgG Stock Solution Needed ( µL) Volume of Unused Eluting Buffer Used ( µL) Total Volume of Solution 0.25 40 210 250  µl 0.50 80 170 250  µl 0.75 120 130 250  µl 1.0 160 90 250  µl 1.5 240 10 250  µl Absorbance Data: Concentrations (mg/mL) Absorbance at 596 nm Set 1 Set 2 Average 1.50 0.608 0.633 0.621 1.00 0.455 0.423 0.439 0.75 0.441 0.246 0.344 0.50 0.162 0.307 0.235 0.25 0.043 0.056 0.050 Unused EB (Control) = 0 0.000 0.000 0.000 Original Flow through 0.004 -0.003 0.001 Washing Buffer 0.002 0.005 0.004 Eluting Buffer 0.001 -0.004 -0.002 Unknown Sample #3 0.608 0.606 0.607 Total Concentration: Protein Sample Concentration (mg/mL) Original Flow through 0.0205 Washing Buffer 0.0274 Eluting Buffer 0.0136 Unknown Sample #3 1.418 GRAPH: CALCULATIONS: The expression given by the best fit linear trend will be used to determine the concentrations for the original flow through, washing flow through, eluting buffer flow through, and unknown sample #3. Where y-represents the absorbance, and x-represents the concentration. The units corresponding to the slope are in mg/mL. Equation of the slope is: y = 0.4336x à ¢Ã‹â€ Ã¢â‚¬â„¢ 0.0079. Solving for x will give us the concentrations of the samples. Concentration of the Original Flow Through: Rearrange to solve for x: y = 0.4336x à ¢Ã‹â€ Ã¢â‚¬â„¢ 0.0079 à ¯Ã†â€™Ã‚ ¨ x = (y 0.0079)/(0.4336) (0.001) = 0.4336x à ¢Ã‹â€ Ã¢â‚¬â„¢ 0.0079 x = (0.001 + 0.0079)/(0.4336) x = 0.0205 mg/mLà ¢Ã¢â‚¬ °Ã‹â€  0.021 mg/mL CALCULATIONS: (continued) Concentration of Washing Buffer Flow Through: y = 0.4336x à ¢Ã‹â€ Ã¢â‚¬â„¢ 0.0079 (0.004) = 0.4336x à ¢Ã‹â€ Ã¢â‚¬â„¢ 0.0079 x = (0.004 + 0.0079)/(0.4336) x = 0.0274 mg/mLà ¢Ã¢â‚¬ °Ã‹â€  0.027 mg/mL Concentration of Eluting Buffer Flow Through: y = 0.4336x à ¢Ã‹â€ Ã¢â‚¬â„¢ 0.0079 (-0.002) = 0.4336x à ¢Ã‹â€ Ã¢â‚¬â„¢ 0.0079 x = (-0.002 + 0.0079)/(0.4336) x = 0.0136 mg/mLà ¢Ã¢â‚¬ °Ã‹â€  0.014 mg/mL Concentration of Unknown Solution #3: y = 0.4336x à ¢Ã‹â€ Ã¢â‚¬â„¢ 0.0079 (0.607) = 0.4336x à ¢Ã‹â€ Ã¢â‚¬â„¢ 0.0079 x = (0.607 + 0.0079)/(0.4336) x = 1.418 mg/mL DISCUSSION: The main goal for this experiment was to determine the protein concentration of the original flow through, washing buffer flow through, eluting buffer flow through, and an unknown sample which was given by the TA. These samples apart from the unknown were prepared in the previous lab experiment and was retained for further analyses for this experiment. In addition to the samples mentioned above, five standard protein solutions were prepared by diluting the 1.56 mg/mL of IgG solution with the unused eluting buffer obtained from lab #6. The concentrations of the standards were as follows: 0.25 mg/mL 0.50 mg/mL, 0.75 mg/mL, 1.0 mg/mL, and 1.5 mg/mL. The unused eluting buffer was also used as the control. It was apparent that upon adding the Bradford reagent to the samples there was visible a color change. A few of the solutions instantly turned to a darker blue hue while others remained a light blue color. The darker color indicated there was a greater concentration of proteins. A calibration curve was generated by plotting the concentrations of the five standards and their respective absorbance reading. It was determined that the best fit for the data was linear which yields an equation in the form of y = mx + b, where y represents the absorbance at 596 nm and x represents the protein concentration. The equation was rearranged as to solve for x and determine the protein concentration of the samples and the unknown #3, based on the data from the averages of the two sets of absorbance measured. However, two values from set two gave negative readings, which was indicative that the concentration of the protein was less than that of the control sample. As per my results, it was concluded that our unknown sample had a total protein concentration of 1.418 mg/mL. The concentrations for the flow through of the original solution, washing buffer, and eluting buffer had a total protein concentration of 0.0205 mg/mL, 0.0274 mg/mL, and 0.0136 mg/mL, respectively. The absorbance data was further analyzed using a 98% confidence interval and yielded a  ± 0.002% margin error. The R-value was relatively close to 1, which gives an equation that is more accurate. This also indicates that the calculated values for the protein concentration will reflect a value closer to the true concentration of the protein of interest. REFERENCES: J. M. Berg, J. L. Tymoczko, G. J. Gatto, Jr., L. Stryer, Biochemistry (8th ed., pp. 70-71). (2015). W.H. Freeman Company. Bradford, M. M. Analytical Biochemistry. Volume 72. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-dye Binding. (pp. 248-254). (1976). Robyt, J. F. and White, B. J. Biochemical Techniques. Theory and Practice. Brooks/Cole, Monterey, CA. (1997) https://www.thermofisher.com/order/catalog/product/23212

Children and Screen Time

Randi Getzelman English 151 03/24/2013 Every parent wants nothing more then their child to succeed. They want their child to develop and learn with out struggle. In this day in age we have access to so many devices that can help contribute to a child’s success. Many will argue that a device such as a I-pad or mobile device will hinder ones learning. I feel the exact opposite will occur, and the child will gain knowledge. Children develop and learn so much at a very young age.They absorb everything they see and hear. They best learn through touch, sight, and sound. Using a device such as a touch I-pad, or I-phone a child can interact on their level. There are so many Apps available for children of all ages. They will help a child develop at their capability and speed, while learning. I feel they will absorb more knowledge with a educational hands on device rather then TV. Children have to interact and use their brains in order for the App to work.Placing a child in front of a T V to just sit and learn, they do not get the same hands on experience. There will be those arguing that placing a phone or tablet in a child’s arms will cause harm. I do not see that being true, in this day in age we use App’s for everything. Rather at the grocery store, banking, or even schools, most teach with touch devices. I feel that helping your child learn with a hands free device, will only help them in the future.

Organizational Leadership and the Role of Self-Evaluation...

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